Coding
El222

Part:BBa_K4427003:Design

Designed by: Quan Luo   Group: iGEM22_JLU_China   (2022-09-20)


El222


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 511
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have used the native light-sensitive DNA-binding protein (EL222) of the Marine bacterium Erythrobacter litoralis HTCC2594 to construct a regulatory primer system (bidirectional regulation) for blue light induction and inhibition in Escherichia coli. EL222 is a modular 222-amino acid photosensitive protein, which is composed of an N-terminal photooxygen voltage (LOV) domain and a C-terminal helix-to-helix (HTH) DNA binding domain, and has the characteristics of a LuxR-type DNA binding protein. Upon exposure to blue light (450 nm), the LOV-HTH interaction is released, enabling EL222 to dimerize and bind to DNA. In the dark, EL222 reverses spontaneously because the N-terminal LOV domain inhibits the C-terminal HTH domain from binding to DNA, thereby rapidly inactivating EL222.

Source

References